Overexpression of GbF3'5'H1 Provides a Potential to Improve the Content of Epicatechin and Gallocatechin.
Identifieur interne : 000240 ( Main/Exploration ); précédent : 000239; suivant : 000241Overexpression of GbF3'5'H1 Provides a Potential to Improve the Content of Epicatechin and Gallocatechin.
Auteurs : Yaqiong Wu [République populaire de Chine, Canada] ; Tongli Wang [Canada] ; Yue Xin [République populaire de Chine] ; Guibin Wang [République populaire de Chine] ; Li-An Xu [République populaire de Chine]Source :
- Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2020.
Abstract
The flavonoids in Ginkgo biloba L. (ginkgo) have important medicinal uses due to their antioxidant, antitumor, and blood circulation-promoting effects. However, the genetic mechanisms underlying flavonoid biosynthesis in ginkgo remain elusive. Flavonoid 3', 5'-hydroxylase (F3'5'H) is an important enzyme in flavonoid synthesis. We detected a novel differentially expressed GbF3'5'H1 gene homologous to the F3'5'H enzyme involved in the flavonoid synthesis pathway through transcriptome sequencing. In this study, we characterized this gene, performed an expression analysis, and heterologously overexpressed GbF3'5'H1 in Populus. Our results showed that GbF3'5'H1 is abundant in the leaf and highly expressed during April. We also found four metabolites closely related to flavonoid biosynthesis. Importantly, the contents of 4',5-dihydroxy-7-glucosyloxyflavanone, epicatechin, and gallocatechin were significantly higher in transgenic plants than in nontransgenic plants. Our findings revealed that the GbF3'5'H1 gene functions in the biosynthesis of flavonoid-related metabolites, suggesting that GbF3'5'H1 represents a prime candidate for future studies (e.g., gene-editing) aiming to optimize ginkgo flavonoid production, especially that of flavan-3-ols.
DOI: 10.3390/molecules25204836
PubMed: 33092253
PubMed Central: PMC7594021
Affiliations:
- Canada, République populaire de Chine
- Colombie-Britannique
- Vancouver
- Université de la Colombie-Britannique
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Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Research Center for Pomology, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Qian Hu Hou Cun No.1, Nanjing 210014, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Center for Pomology, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Qian Hu Hou Cun No.1, Nanjing 210014</wicri:regionArea><wicri:noRegion>Nanjing 210014</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC V6T 1Z4</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Wang, Tongli" sort="Wang, Tongli" uniqKey="Wang T" first="Tongli" last="Wang">Tongli Wang</name><affiliation wicri:level="4"><nlm:affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC V6T 1Z4</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Xin, Yue" sort="Xin, Yue" uniqKey="Xin Y" first="Yue" last="Xin">Yue Xin</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Guibin" sort="Wang, Guibin" uniqKey="Wang G" first="Guibin" last="Wang">Guibin Wang</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Li An" sort="Xu, Li An" uniqKey="Xu L" first="Li-An" last="Xu">Li-An Xu</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecules (Basel, Switzerland)</title><idno type="eISSN">1420-3049</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The flavonoids in <i>Ginkgo biloba</i> L. (<i>ginkgo</i>) have important medicinal uses due to their antioxidant, antitumor, and blood circulation-promoting effects. However, the genetic mechanisms underlying flavonoid biosynthesis in <i>ginkgo</i> remain elusive. Flavonoid 3', 5'-hydroxylase (F3'5'H) is an important enzyme in flavonoid synthesis. We detected a novel differentially expressed <i>GbF3'5'H1</i> gene homologous to the F3'5'H enzyme involved in the flavonoid synthesis pathway through transcriptome sequencing. In this study, we characterized this gene, performed an expression analysis, and heterologously overexpressed GbF3'5'H1 in <i>Populus</i>. Our results showed that <i>GbF3'5'H1</i> is abundant in the leaf and highly expressed during April. We also found four metabolites closely related to flavonoid biosynthesis. Importantly, the contents of 4',5-dihydroxy-7-glucosyloxyflavanone, epicatechin, and gallocatechin were significantly higher in transgenic plants than in nontransgenic plants. Our findings revealed that the <i>GbF3'5'H1</i> gene functions in the biosynthesis of flavonoid-related metabolites, suggesting that GbF3'5'H1 represents a prime candidate for future studies (e.g., gene-editing) aiming to optimize <i>ginkgo</i> flavonoid production, especially that of flavan-3-ols.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">33092253</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>31</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1420-3049</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>20</Issue><PubDate><Year>2020</Year><Month>Oct</Month><Day>20</Day></PubDate></JournalIssue><Title>Molecules (Basel, Switzerland)</Title><ISOAbbreviation>Molecules</ISOAbbreviation></Journal><ArticleTitle>Overexpression of <i>GbF3'5'H1</i> Provides a Potential to Improve the Content of Epicatechin and Gallocatechin.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E4836</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/molecules25204836</ELocationID><Abstract><AbstractText>The flavonoids in <i>Ginkgo biloba</i> L. (<i>ginkgo</i>) have important medicinal uses due to their antioxidant, antitumor, and blood circulation-promoting effects. However, the genetic mechanisms underlying flavonoid biosynthesis in <i>ginkgo</i> remain elusive. Flavonoid 3', 5'-hydroxylase (F3'5'H) is an important enzyme in flavonoid synthesis. We detected a novel differentially expressed <i>GbF3'5'H1</i> gene homologous to the F3'5'H enzyme involved in the flavonoid synthesis pathway through transcriptome sequencing. In this study, we characterized this gene, performed an expression analysis, and heterologously overexpressed GbF3'5'H1 in <i>Populus</i>. Our results showed that <i>GbF3'5'H1</i> is abundant in the leaf and highly expressed during April. We also found four metabolites closely related to flavonoid biosynthesis. Importantly, the contents of 4',5-dihydroxy-7-glucosyloxyflavanone, epicatechin, and gallocatechin were significantly higher in transgenic plants than in nontransgenic plants. Our findings revealed that the <i>GbF3'5'H1</i> gene functions in the biosynthesis of flavonoid-related metabolites, suggesting that GbF3'5'H1 represents a prime candidate for future studies (e.g., gene-editing) aiming to optimize <i>ginkgo</i> flavonoid production, especially that of flavan-3-ols.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Yaqiong</ForeName><Initials>Y</Initials><Identifier Source="ORCID">0000-0002-7101-4413</Identifier><AffiliationInfo><Affiliation>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Center for Pomology, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Qian Hu Hou Cun No.1, Nanjing 210014, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Tongli</ForeName><Initials>T</Initials><Identifier Source="ORCID">0000-0002-9967-6769</Identifier><AffiliationInfo><Affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xin</LastName><ForeName>Yue</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Guibin</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Li-An</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>31971689</GrantID><Agency>National Natural Science Fundation of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Molecules</MedlineTA><NlmUniqueID>100964009</NlmUniqueID><ISSNLinking>1420-3049</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">F3′5′H</Keyword><Keyword MajorTopicYN="N">flavonoid</Keyword><Keyword MajorTopicYN="N">metabolite</Keyword><Keyword MajorTopicYN="N">overexpression</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>09</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>16</Day></PubMedPubDate><PubMedPubDate 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IdType="pubmed">13130383</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>République populaire de Chine</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Yaqiong" sort="Wu, Yaqiong" uniqKey="Wu Y" first="Yaqiong" last="Wu">Yaqiong Wu</name></noRegion><name sortKey="Wang, Guibin" sort="Wang, Guibin" uniqKey="Wang G" first="Guibin" last="Wang">Guibin Wang</name><name sortKey="Wu, Yaqiong" sort="Wu, Yaqiong" uniqKey="Wu Y" first="Yaqiong" last="Wu">Yaqiong Wu</name><name sortKey="Xin, Yue" sort="Xin, Yue" uniqKey="Xin Y" first="Yue" last="Xin">Yue Xin</name><name sortKey="Xu, Li An" sort="Xu, Li An" uniqKey="Xu L" first="Li-An" last="Xu">Li-An Xu</name></country><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Wu, Yaqiong" sort="Wu, Yaqiong" uniqKey="Wu Y" first="Yaqiong" last="Wu">Yaqiong Wu</name></region><name sortKey="Wang, Tongli" sort="Wang, Tongli" uniqKey="Wang T" first="Tongli" last="Wang">Tongli Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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